May Parker
May Parker (better known as "Aunt May") was the guardian for young Peter Parker after his parents were killed. After her husband Ben was killed by a burglar, May raised Peter as her own child, and still frets over her nephew to this day.
Aunt May has been considered dead twice, survived numerous near-fatal periods of bad health, dated Willie Lumpkin and Edwin Jarvis, almost married Dr. Octopus, and became a herald of Galactus named Golden Oldie. Despite everything, she still has a positive outlook on life and is a huge source of strength for Peter.